Assistants Martin Raymond and Dan Lacroix will share coaching responsibilities for Sunday night's game at Winnipeg.
"Guy has poured his heart and soul into the Lightning organization for these past three years and we appreciate all the work he has done," general manager Steve Yzerman said in a statement. "But ultimately I am not satisfied with the direction we are heading and I believe making a change today is in the best interest of our franchise."
Tampa Bay is next to last in the Eastern Conference with a 13-17-1 record. Boucher was hired as the Lightning's seventh coach on June 10, 2010.
Boucher's dismissal came one day after the Lightning fell behind by four goals in the first period of a 5-3 loss at Ottawa.
The Lightning appear headed toward missing the playoffs for the second consecutive season after reaching the Eastern Conference finals during Boucher's first year.
Tampa Bay entered this season with postseason aspirations after adding goalie Anders Lindback and defensemen Sami Salo and Matt Carle. Lindback and team captain Vincent Lecavalier have been among the players sidelined by injuries.
Boucher's hiring was Yzerman's first major move as GM. Boucher had only one year of professional coaching experience, none on hockey's highest level. But Yzerman was not deterred from making him the league's youngest coach, saying the 38-year-old had adapted to the players, personalities and level of play he encountered at every stage of his career.
Boucher was a junior league coach for three years before taking over the American Hockey League's Hamilton Bulldogs, Montreal's top minor league affiliate. He led Hamilton to the second-best record in the AHL then turned down a chance to coach the Columbus Blue Jackets.
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