The trio will be replaced by D.C. United defenders Bobby Boswell and Sean Franklin, as well as Philadelphia Union midfielders Maurice Edu, All-Star coach Caleb Porter announced Saturday.
The All-Star match is set for Wednesday night at Providence Park, home of the Portland Timbers.
Keane and Gonzalez would have been limited because the Galaxy play the San Jose Earthquakes in a match on Friday, Porter said.
Galaxy forward Landon Donovan, making his record 14th All-Star appearance, will remain on the roster.
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